Articles — Project / Lab Report

Report on laboratory practical

Esse trabalho tem como objetivo formular problemas como busca.

rapport final

Bacterial cells can have DNA damage due to transcriptional error, or through the effect of an antibiotic. The SOS response is a bacterial cell program for coping with DNA damage, in which the cell cycle is arrested, and DNA repair is induced. The repairs have high probability in leading to mutagenesis in the bacteria, which can lead to antibiotic resistance. The RecA protein in bacteria is responsible for the activation of the SOS response; therefore, making it a target for inhibition. I elected to use the ubiquitination system, natively used for apoptosis, as a means of targeted degradation of the RecA protein in bacteria prone to mutations. Polyubiquitination of misfolded proteins leads to the breaking down of the protein with the aid of proteasomes, which break down unnecessary proteins through a chemical reaction known as proteolysis. Using random forest-predictors, I determined a statistically high likelihood of ubiquitination of the RecA protein in MRSA, Tuberculosis, and other high risk bacterial infections. I hypothesized that I could foster ubiquitin-tagging on RecA by forcing the protein to misfold. Chaperones are proteins which interact with each other to prevent specific sets of proteins from misfolding. CHIP (C terminus of HSC70-Interacting Protein) is a biomolecule that inhibits interactions between the chaperones of RecA. Adding CHIP, ubiquitin, and 26s proteasomes into the bacterial system, should theoretically lead to the degradation of the RecA protein inside the bacteria. I tested my hypothesis by conducting an assay for monitoring CHIP-mediated ubiquitination, and conducted analysis on the assay using SDS- Page gel electrophoresis, and Western-blotting. The resulting data showed signs of polyubiquitination on the RecA protein, with chains of five or more ubiquitin, showing high drug potential. Adding an antibody drug conjugate, containing all the necessary components of a CHIP-mediated ubiquitination reaction, to common antibiotics can lead to the inhibition of bacterial mutagenesis, and higher antibiotic drug potency.

This investigation explores the conditions under which an object, moving through a liquid in high speed, has the greatest deceleration. The conditions focused on are the mass of the object, its initial velocity and its entry angle into the liquid. This is achieved through shooting pellets of varied masses and energies out of two different soft guns at a water container, recording them with a high speed camera and mapping their motion with a tracking program. The results of the experiment indicate that the lesser the mass of the object, the greater its deceleration in the fluid. The initial velocity and the entry angle of the object do not influence the rate of its speed loss. It is further explored that as the density of the fluid becomes greater, so does the deceleration of the object. The investigation is intended to deepen the understanding of the high speed motion through a medium.

This is a simple lab report version about strain gauge, which contains graphs, tables, and formula.

En el siguiente informe se muestran los datos obtenidos en el laboratorio numero 1 de circuitos y dispositivos, el cual se basa en circuitos en serie y paralelo, y cuales son sus voltajes corrientes y potencias.

ERCESI Weekly Report template.

his project is part of the FlowNet initiative. FlowNet aims at providing Internet freedom and free flow information through socially informed, censor resistant online social networks. My contribution for FLowNet is in devel- oping an Android application, SecurePost. The requirement for SecurePost is two-fold. First, the system should facilitate secure, anonymous, group communication within a closed group of trusted members. Second, the general public on the Internet viewing this content, should be able to verify that the content was generated only by the said closed group of trusted members. The system consists of an Android client application, a proxy server and a browser-plugin. The OSNs supported by this system are Twitter and Facebook.